Project Design

Inclusion and Exclusion Criteria

Work Package 1 – Synovial Joint Fluid Collection, Storage and Preparation for Analysis

Deliverables of WP1:

• 1.1. A protocol for collecting synovial joint fluid from patients will be designed and published as technical information on the official web page.
• 1.2. A protocol for the preparation of synovial joint fluid for analysis will be desgned and published as technical information on the official web page.
• 1.3. Synovial joint fluid storage protocol will be desgned and published as technical information on the official web page.

Work Package 2 – Synthesis of IMAC Sorbent for Phosphopeptide Enrichment

Deliverables of WP2:

• 2.1. Methods and images for the development and characterization of immobilized metal affinity chromatography (IMAC) sorbents in microsphere form will be published as technical information on the official web page.
• 2.2. The method for phosphopeptide enrichment in reference standard protein and synovial fluid will be published as technical information on the official web page.
• 2.3. The data obtained from the investigation of the phosphopeptide isolation performance of microspheres by mass spectrometry will be stored to be used in the article and presented at the congress. It is planned to make a presentation at the congresses between 12-24 months. Additionally, publish an article on sorbent synthesis is planned within 12-24 months.
• 2.4. When the phospholipid isolation performance of microspheres is compared with commercial kits, a result similar to and/or better than the commercial kits (based on matching criteria in terms of peak number and commercial mass profile) is obtained, this data will be published in reputable scientific journals “sample preparation – material technology” between 12-24 months. It is planned to be published in special issues on the subject.

Work Package 3 – Metabolomic, Lipidomic and Proteomic Analyzes

Deliverables of WP3:

• 3.1. The protocol for examining joint fluid with omic technology will be published on the official web pageas technical information.
• 3.2. After the omic analyzes are completed, a brochure will be printed and a bulletin will be published. Information will also be announced via social media. A workshop on omics analysis of joint fluid will be planned and will be linked to the CA21110 COST Action.
• 3.3. Omics analysis results from synovial fluid will be presented at the scientific congress and then an article will be published.

Work Package 4 – Reliability Assessment with ELISA

Deliverables of WP4:

• 4.1. The amounts of inflammation markers determined in the joint fluid will be present as mean and standard deviation and presented as graphs. A protocol for the analysis of joint fluid in the Multiplex ELISA system will be designed and published as technical information on the official web page. The information obtained will be presented at scientific congresses, meetings and workshops.
• 4.2. The amounts of cartilage formation and destruction markers determined in the joint fluid will be present as mean and standard deviation and presented as graphs. The method will be published as a technical note on the official web page and meaningful data will be presented at congresses and reflected in the article.
• 4.3. A scientific article will be published after ELISA and omics data are compared and interpreted. A link to this publication will be provided on the official web page after the publication. Broadcast information will be announced through media and social media. The final output of the project will be shared with stakeholders at the COST-Action CA21110 regular meetings. A training school and workshop will be organized for ELISA study. One doctoral and one master’s scholar will be trained in the techniques and technologies used. If originality can be found in the process or result, a national patent application will be made.

Educational Videos

1-Mass Spectrometry in biochemistry

2-Diagnostic and Treatment Monitoring Methods with LC-MS based Metabolomics Approaches

3-The Process from GC-MS based Metabolomics Studies to Biosensors for Personalized Medicine Applications

4-Development of a Novel In Vitro Mitophagy Model using the Bacterial HOK Protein

5-Peptidyl-Prolyl Cis-Trans Isomerase Pin1: From Cellular Functions to Multiple Omics Approaches

6-Utilizing the Power of Biological Biotinylation to Investigate Membrane Proteomes of Breast Cancer Cell Lines